Journal: Nucleic Acids Research

Article Title: NSrp70 is a lymphocyte-essential splicing factor that controls thymocyte development

doi: 10.1093/nar/gkab389

Figure Lengend Snippet: Deletion of NSrp70 results in defective survival signals following TCR activation in CD69 + DP thymocytes. ( A ) KEGG T cell receptor signaling pathway. The blue asterisks (*) represent down-regulated genes from the RNA-seq analysis (Figure ). ( B ) Expression of TCRβ, CD3ϵ, and CD3ζ on CD69 + DP thymocytes from Nsrp1 f/f (WT) and Nsrp1 f/f CD4Cre (KO) mice. ( C ) A schematic model of gene regulation by NSrp70. NSrp70 sequesters splicing factors in the nuclear speckles. Disintegration of splicing factors by NSrp70 deletion induces abnormal gene regulation during thymocyte development. As one of the results, reduced TCR expression may cause impaired T cell maturation. ( D ) Calcium flux in DP thymocytes. Cells from (A) were stimulated with PMA and ionomycin (P/I) or anti-CD3/CD28 antibodies, and then calcium fluxes were measured by flow cytometry. ( E ) Western blot of ZAP70, PKCθ, Erk1/2, and p38 in DP cell lysates stimulated on anti-CD3/28 for 0, 5, and 20 min. β-actin served as the loading control. M, molecular mass (KDa). ( F ) In vitro thymocyte development assay. CD69 – DP thymocytes were stimulated on anti-TCRβ/CD2 antibodies for 20 h (stimulation), or the cells were further incubated for 20 h in medium without stimulation (recovery). (G and H) Cells from (F) were stained for Ki-67 ( G ) or annexin V and 7ADD ( H ). Cells were analyzed by flow cytometry (F–H). The bar graphs indicate mean fluorescence intensities (MFI) (G). *, meaningful P -value; NC, non-coated; S, stimulation; R, recovery. The bar graphs indicate average ± standard deviation of apoptotic and dead thymocytes population (H). NS, non-significant P -value. All data shown are representative of three independent experiments.

Article Snippet: Anti-CD2 antibody (130-100-617) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).

Techniques: Activation Assay, RNA Sequencing, Expressing, Flow Cytometry, Western Blot, Control, In Vitro, Incubation, Staining, Fluorescence, Standard Deviation

Journal: Molecules (Basel, Switzerland)

Article Title: Effect of Supplementation with the Combination of Se-Enriched Lentinula edodes Mycelium, Exogenous Enzymes, Acidifiers, Sodium Butyrate and Silicon Dioxide Nanoparticle Feed Additives on Selected Parameters in Calves.

doi: 10.3390/molecules27165163

Figure Lengend Snippet: Figure 2. Percentage of the lymphocyte (LYM) subpopulations containing surface antigens in the peripheral blood of experimental (E) and control (C) calves: (a) CD2+; (b) CD4+; (c) CD8+; (d) WC1+; (e) MHC class II, and (f) percentage of the monocyte (MON) subpopulation containing CD11b+

Article Snippet: The following mouse anti-bovine monoclonal antibodies (mAb) with fluorescein isothiocyanate (FITC) were used: CD2+, CD4+, CD8+, WC1+, MHC class II monomorphic, and CD11b+ (Bio-Rad, Hercules, USA).

Techniques: Control